AG - Eva Bartok

Dr. med. Eva Bartok
Institut für Klinische Chemie und Klinische Pharmakologie
Universitätsklinikum Bonn
Biomedizinisches Zentrum
Sigmund-Freud-Str. 25
53127 Bonn


Tel: 0228-287-51167
Fax: 0228-287-16094

E-mail: ebartok@uni-bonn.de
Lebenslauf: bartok.pdf


 
 

Research

Our research focuses on the participation of non-immune cells in innate immune sensing, both via activation of cell-intrinsic receptors and through crosstalk with professional immune cells.

Innate immune sensing is vital for the survival of all multicellular organisms, whether plants, drosophila or humans. In sickness and in health, our bodies are under the constant surveillance of innate immune cells, which search for signs of “danger” from within and without. To do so, they make use of a repertoire of germ-line encoded receptors, known as pattern-recognition receptors or PRRs, which detect molecular patterns that are associated with cellular damage or microbial pathogens (Matzinger, 1994; 2002; Medzhitov & Janeway, 2000).

Initially, innate immune sensing was thought to be restricted to a particular class of professional immune cells, including neutrophils, monocytes, macrophages and dendritic cells. Indeed, these cells express the largest variety of innate immune receptors and are critical for in the direct elimination of pathogens and the recruitment of adaptive immune cells. In contrast, non-immune cells have been traditionally viewed as bystanders to this process, passively enduring damage, infection and, if necessary, elimination without actively contributing to host defense. 

However, in recent years, we have come to understand that almost all cells are capable of some form of innate immune sensing. Certain PRRs, such as RIG-I and MDA-5, which detect cytosolic dsRNA, are expressed almost ubiquitously while others, such as TLR 7, which senses endosomal ssRNA, are expressed restrictively in specific immune cell populations (Barchet, Wimmenauer, Schlee, & Hartmann, 2008; Junt & Barchet, 2015). Since most pathogens are molecularly complex and capable of activating several PRRs simultaneously, this cell-specific distribution of innate immune sensors allows for differential roles in the immune response for distinct cellular populations. 

We would like to elucidate how non-myeloid somatic cells participate in the immune sensory system, both via direct innate immune sensing and in their capacity to influence traditional innate immune cells within the host.  We hope that a better understanding of these interactions will also shed more light on the role of the innate immune sensing in pathogen defense and autoinflammatory disease.


Figure 1 (a): Patient epithelial cells cultivated in our lab. 

        

Immune Sensing in Adenoid and Nasal Tissue

Everything we ingest or inhale passes through our nasopharygeal space on its way into our bodies. Thus, the adenoid and tonsillar lymphatic tissue serve as a particularly important immune checkpoint in our bodies. Moreover, since the nasopharyngeal space is not sterile, even under physiological conditions, immune cells in this environment face special challenges distinguishing between normal microbial exposition and pathogenic microbiota. In collaboration with Stephan Herbehold and Evelyn Hartmann from the ENT department the University Hospital (HNO/UKB), we are investigating the behavior of myeloid immune cells within adenoids and the influence of adenoid epithelia on the innate immune response.  

        

 

 






 

























Figure 1 (b): Epithelial cells are capable of immune signaling. Figure 1 (c): CD14+ cells from the blood differentially regulate their immune response
after exposed to adenoid epithelial cell-treated medium.

 
 








Figure 1 (d): CD14+ cells from the blood differentially regulate their immune response
after exposed to adenoid epithelial cell-treated medium.
Figure 1 (e): CD14+ cells from the blood differentially regulate their immune response
after exposed to adenoid epithelial cell-treated medium.

 

 

Nucleic Acid Sensing in the Central Nervous System

Immune defense is necessary to maintaining the integrity and function of the central nervous system. Nonetheless, an excessive immune response within the CNS can also inflict irreparable damage.  In collaboration with Jonas Doerr and Oliver Brüstle (Institute for Reconstructive Neurobiology), we are currently investigating the cell-intrinsic innate immune sensing in induced pluripotent stem-cell derived (iPSC) neurons.  Our focus is on nucleic acid recognition in CNS-resident cells and its role in host defense.

 


































Figure 2 (a): The live spread of fluorescently marked RABV through neurons in the murine brain Image courtesy of Jonas Doerr, Institute of Reconstructive Neurobiology. 

 



The rabies virus is an excellent example for the importance of the differential cell-intrinsic response. Rabies virus (RABV) is a Group V (-)ssRNA virus, and its purified genome can be detected by TLR3 and RIG-I(Hornung et al., 2006; Schlee, 2013), which both lead to induction of Type-I interferon. Nonetheless, TLR3, which is predominantly expressed on microglia(Town, Jeng, Alexopoulou, Tan, & Flavell, 2006), has been shown to lead to deleterious immune hyperreactivity and increase neuropathology (Carty, Reinert, Paludan, & Bowie, 2014; Ménager et al., 2009), while RIG-I, which is expressed in all CNS cells, has a positive effect on viral clearance(Carty et al., 2014; Rieder & Conzelmann, 2011). Underscoring the importance of RIG-I sensing of RABV, RABV has evolved measures to suppress RIG-I-mediated immunosensing, such as RABV P protein(Rieder & Conzelmann, 2011; Schlee, 2013). Since RABV is neurotropic, cell-intrinsic RIG-I mediated RABV recognition in neurons may be the central mechanism to establishing an effective immune response against an otherwise fatal pathogen.

 
        











Figure 2 (b): iPSC-Neurons react potently to 5’-triphosphate RNA, a canonical RIG-I ligand.

 

 

References

Barchet, W., Wimmenauer, V., Schlee, M., & Hartmann, G. (2008). Accessing the therapeutic potential of immunostimulatory nucleic acids. Current Opinion in Immunology, 20(4), 389–395. http://doi.org/10.1016/j.coi.2008.07.007

Carty, M., Reinert, L., Paludan, S. R., & Bowie, A. G. (2014). Innate antiviral signalling in the central nervous system. Trends in Immunology, 35(2), 79–87. http://doi.org/10.1016/j.it.2013.10.012

Hornung, V., Ellegast, J., Kim, S., Brzózka, K., Jung, A., Kato, H., et al. (2006). 5'-Triphosphate RNA is the ligand for RIG-I. Science, 314(5801), 994–997. http://doi.org/10.1126/science.1132505

Junt, T., & Barchet, W. (2015). Translating nucleic acid-sensing pathways into therapies. Nature Publishing Group, 15(9), 529–544. http://doi.org/10.1038/nri3875

Matzinger, P. (1994). Tolerance, danger, and the extended family. Annual Review of Immunology, 12(1), 991–1045. http://doi.org/10.1146/annurev.iy.12.040194.005015

Matzinger, P. (2002). The Danger Model: A Renewed Sense of Self. Science, 296(5566), 301–305. http://doi.org/10.1126/science.1071059

Medzhitov, R., & Janeway, C. (2000). Innate immune recognition: mechanisms and pathways. Immunological Reviews, 173, 89–97.

Ménager, P., Roux, P., Mégret, F., Bourgeois, J.-P., Le Sourd, A.-M., Danckaert, A., et al. (2009). Toll-Like Receptor 3 (TLR3) Plays a Major Role in the Formation of Rabies Virus Negri Bodies. PLoS Pathogens, 5(2), e1000315–15. http://doi.org/10.1371/journal.ppat.1000315

Rieder, M., & Conzelmann, K.-K. (2011). Chapter 6 - Interferon in Rabies Virus Infection. Rabies Research (1st ed., Vol. 79, pp. 91–114). Elsevier Inc. http://doi.org/10.1016/B978-0-12-387040-7.00006-8

Schlee, M. (2013). Master sensors of pathogenic RNA – RIG-I like receptors. Immunobiology, 218(11), 1322–1335. http://doi.org/10.1016/j.imbio.2013.06.007

Town, T., Jeng, D., Alexopoulou, L., Tan, J., & Flavell, R. A. (2006). Microglia Recognize Double-Stranded RNA via TLR3. The Journal of Immunology, 176(6), 3804–3812. http://doi.org/10.4049/jimmunol.176.6.3804

 
 

Mitarbeiter

Die Vorwahl für das Institut ist +49 (0) 228 - 287 -, dann wählen Sie bitte die Nummer des gewünschten Mitarbeiters.

Name Vorname Titel Funktion Durchwahl e-Mail
Hadaddzadeh Shakiba Mehrnoush   PhD 51626 shakiba.mehrnoush@ukbonn.de
Soltész Leon   Med. Doktorand/SHK 51626 s4lesolt@uni-bonn.de
Nastaly Maximilian   Med. Doktorand/SHK 51511 s4manast@uni-bonn.de
Ostendorf Thomas   Med. Doktorand 51511 s4thoste@uni-bonn.de
Schmitz Saskia   MTA 51626 saskia.schmitz@ukbonn.de
Zuber Sarah   SHK 51511  




 































von links nach rechts: Dr. med. Eva Bartok, Maximilian Nastaly, Charikleia Kazila






Information for Students

Motivated students interested in pursuing a medical thesis (Dr. med) in our laboratory are encouraged to apply!  We are looking for students with a genuine interest in immunology, who would like to learn the basics of practical laboratory research. In general, students should be willing to apply for a scholarship from BONFOR or the Else Kröner-Fresenius Foundation and take a leave of absence of at least one semester from their studies.





Publikationen

 

Stand: 25. Januar 2018