Cell Programming Unit - Our Services

 

The CPU employs state-of-the-art cell reprogramming and differentiation technologies for CNS-related research purposes and is constantly refining its technological portfolio. CPU staff has long-standing expertise in the set-up of disease models of neurological disorders.

To promote the establishment of innovative stem cell-based in vitro models we offer the following services:

 

Generation of iPS cell lines
As starting material for iPS cell derivation we accept skin fibroblasts or peripheral blood mononuclear cells (PBMCs). Donor cells are reprogrammed using the latest Sendai virus-based reprogramming vectors that guarantee transient introduction of transgenes to avoid the stable integration of reprogramming factors. As a standard procedure, we isolate and expand up to five clonal iPS cell lines per subject for cryopreservation and subsequent shipping to the investigator. Depending on the recipients’ demands, selected clones are subjected to standardized quality control regimens (ICC and gene expression profiling for pluripotency analysis, assessment of transgene removal, STR analysis, SNP genotyping). Please consult our detailed price list for current rates.

 

Neural differentiation of iPS cells for disease modeling
The Cell Programming Unit has in-depth expertise in the differentiation of iPS cells into stable neural precursors as well as terminally differentiated neural cells (e.g. cortical neurons, motoneurons, dopamine neurons and glial subtypes). We provide consulting support to interested researchers and, in selected cases, the provision of neural subtypes for collaborative projects aiming at modelling neurological disorders, high content analyses and drug development.